Experience – Anurag Kumar Srivastava

Thesis title: “Applying meta-analysis to develop novel anti-tumor strategies.” (read detail summary of my work here)
Conducted integrative analysis of adiponectin receptor expression in a dataset of 10,000 cancer patients, uncovering insights into survival outcomes.
Discovered and demonstrated that lower expression of adiponectin receptors correlates with improved survival across multiple cancer types.
Authored and secured a competitive research grant for my Ph.D., showcasing strong proposal writing and research skills.
Skills: Data Visualization, Bioinformatics, Cancer Biology, Obesity, Type 2 Diabetes

Facilitated an international collaboration to investigate the role of adiponectin and its receptors in pancreatic cancer.
Worked with a cross-functional team of statisticians, bioinformaticians, clinicians, and engineers.
Demonstrated the role of adiponectin in pancreatic cancer progression via activation of pro-tumorogenic signals.
Skills: Bioinformatics, Oncology, Big Data Analysis, Survival Analysis, Immunology, Metabolism, Obesity, Type 2 Diabetes

Tested for antibodies against acetylated sites of enolase in serum samples from 100+ pancreatic cancer patients.
Investigated the role of post-translational modifications (PTMs) in tumor-associated antigens (TAA) and their implications in cancer.
Skills: Immunology, Protein Biology, Biochemistry, Cancer Biology, Immunotherapy, Molecular Biology, RNA interference, Cell Biology, FACS

Genetically incorporated post-translational modifications into the glycolytic enzyme enolase using genetic code expansion technology.
Purified protein samples using size exclusion chromatography for further analysis.
Confirmed incorporation of unnatural amino acids via Western blotting and mass spectrometry.
Skills: Biochemistry, Structure Biology, Chemical Biology, Cancer Biology, Metabolism,

Studied the role of H3K36 methylation in radiation resistance in glioblastoma (GBM) cells, focusing on DNA Damage Repair (DDR) pathway proteins.
Performed Western blotting to analyze DDR protein overexpression and Comet Assay to assess DNA damage.
Found that relapse GBM populations exhibited no DNA damage, suggesting rapid DDR activity.
Skills: Cell Biology, Neurobiology, Tumor Biology, Epigenetics,

Developed a tunable Genetic Code Expansion (GCE) system for incorporating multiple unnatural amino acids into proteins to study protein dynamics.
Optimized protein expression using the GCE system and purified the expressed proteins via size exclusion chromatography.
Cultured microorganisms to facilitate the production of proteins with unnatural amino acids.
Work selected as the best poster in the biotechnology category at an international conference in India.
Skills: Molecular Biology, Cell Biology, Microbiology, Protein Biology, Epigenetics

Investigated ATP generation via the Arginine Deiminase (ADI) pathway in lactic acid bacteria as a potential fuel source for self-sustaining synthetic cells.
Cloned and optimized expression of the four genes involved in the ADI pathway.
Characterized Carbamate Kinase, focusing on its enzymatic activity and stability.
Skills: Cloning, Enzyme Assay, Microbiology, Protein Biology, Biochemistry

Cultured microbial strains for infectious diseases
Won the thrid prize at national conference in India for paper presented from this work
Skills: Infectious Diseases, Microbiology, Fermentation

Performed competitive and content audits for clients like IgG4ward!, Baxter, Arralyze, Qbench and others
Wrote impactful diverse contents like website copies, case studies, blogs, white papers, eBooks, infographics, flyers and other marketing collaterals
Spoke on podcasts addressing academic toxicity
Co-hosted webinar on Gender Equity in STEM
Collaborated globally with science communicators on topics like climate change, mental health, and air pollution.
Delivered high-quality content within specified timelines.
Demonstrated adaptability by tackling different scientific subjects and effectively communicating diverse topics.

Oversaw the comprehensive management of the company’s LinkedIn page, successfully achieving a 30% increase in followers and enhancing overall engagement by 30%.
Authored impactful content, leading to a notable 10-20% increase in website traffic.
Crafted case studies and whitepapers that directly contributed to service sales amounting to $50K-75K.
Penned diverse articles covering a broad spectrum of topics, including single-cell analysis, bulk RNA, metabolomics, proteomics, and non-omics datasets, as well as discussions on obesity, rare diseases, metabolic disorders, autoimmune disorders, and others.
Actively collaborated in cross-functional teams, playing a key role in enhancing organizational efficiency in project execution.

Performed an economic assessment of current surveillance systems for pandemic preparedness, zoonoses, and antimicrobial resistance prevention.
Formulated recommendations for priority investment that will be included in the Central Asia One Health Regional Framework for Action.
Developed quality assurance processes, protocols, and appraisal rating systems to measure the effectiveness of programs and projects.

Authored educational articles on diverse topics including exosomes, epigenetics, neurobiology, women reproductive health, sleep disorders, degenerative disorders, autoimmune diseases,and aging for platforms like Troscriptions, IndiaBioSciences, and Minnac Life Sciences.
Wrote personal narratives addressing academic toxicity and myths about international applications, fostering awareness and advocacy within the scientific community.
Contributed to science journalism during an internship with Gilead Amit (The Economist), producing an article on farmed fish welfare published in the NASW magazine.
Drafted grant proposals for projects on infectious diseases, neglected tropical diseases, and pandemic preparedness, demonstrating persuasive writing skills.
Translated complex concepts into accessible content, bridging the gap between cutting-edge science and diverse audiences.

Delivered exceptional results, with clients able to secure grants exceeding USD 4 million across 25+ countries.
Empowered and prepared over 3000 students for successful international applications in 15 different countries.
Provided hands-on mentoring in application drafting, interview preparation, and comprehensive assistance in grant proposal writing, manuscript preparation, and thesis drafting.
Witnessed clients achieve placements in the top 100 universities globally, with prestigious scholarships like Chevening, MITACS, Harvard (link), Darwin Trust (link),Hong Kong Ph.D. Fellowship Scheme, and more.